Title | Ultra-bright and -stable red and near-infrared squaraine fluorophores for in vivo two-photon imaging. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Podgorski K, Terpetschnig E, Klochko OP, Obukhova OM, Haas K |
Journal | PLoS One |
Volume | 7 |
Issue | 12 |
Pagination | e51980 |
Date Published | 2012 |
ISSN | 1932-6203 |
Keywords | Animals, Cyclobutanes, Fluorescent Dyes, Microscopy, Fluorescence, Multiphoton, Nerve Tissue Proteins, Neurons, Phenols, Photons, Rotaxanes, Synapses, Xenopus laevis |
Abstract | Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95. |
URL | https://www.ncbi.nlm.nih.gov/pubmed/23251670 |
DOI | 10.1371/journal.pone.0051980 |
Alternate Journal | PLoS ONE |
PubMed ID | 23251670 |
PubMed Central ID | PMC3522634 |
Grant List | / / Canadian Institutes of Health Research / Canada |