Title | The scaffold protein, Homer1b/c, regulates axon pathfinding in the central nervous system in vivo. |
Publication Type | Journal Article |
Year of Publication | 2001 |
Authors | Foa L, Rajan I, Haas K, Wu GY, Brakeman P, Worley P, Cline H |
Journal | Nat Neurosci |
Volume | 4 |
Issue | 5 |
Pagination | 499-506 |
Date Published | 2001 May |
ISSN | 1097-6256 |
Keywords | Animals, Axons, Blotting, Western, Carrier Proteins, Central Nervous System, Electroporation, Heterozygote, Homer Scaffolding Proteins, Image Processing, Computer-Assisted, Immunohistochemistry, Ligands, Neuropeptides, Oocytes, Organ Culture Techniques, Rats, Signal Transduction, Superior Colliculi, Vaccinia virus, Xenopus |
Abstract | Homer proteins are a family of multidomain cytosolic proteins that have been postulated to serve as scaffold proteins that affect responses to extracellular signals by regulating protein-protein interactions. We tested whether Homer proteins are involved in axon pathfinding in vivo, by expressing both wild-type and mutant isoforms of Homer in Xenopus optic tectal neurons. Time-lapse imaging demonstrated that interfering with the ability of endogenous Homer to form protein-protein interactions resulted in axon pathfinding errors at stereotypical choice points. These data demonstrate a function for scaffold proteins such as Homer in axon guidance. Homer may facilitate signal transduction from cell-surface receptors to intracellular proteins that govern the establishment of axon trajectories. |
URL | https://www.ncbi.nlm.nih.gov/pubmed/11319558 |
DOI | 10.1038/87447 |
Alternate Journal | Nat. Neurosci. |
PubMed ID | 11319558 |