Fast non-negative temporal deconvolution for laser scanning microscopy.

TitleFast non-negative temporal deconvolution for laser scanning microscopy.
Publication TypeJournal Article
Year of Publication2013
AuthorsPodgorski K, Haas K
JournalJ Biophotonics
Date Published2013 Feb
KeywordsAlgorithms, Animals, Calcium Signaling, Computer Simulation, Fluorescent Dyes, Microscopy, Confocal, Microscopy, Fluorescence, Multiphoton, Molecular Imaging, Neurons, Optical Phenomena, Signal Processing, Computer-Assisted, Superior Colliculi, Xenopus laevis

Laser scanning microscopy (LSM) is a common technique for high resolution fluorescent imaging. Here we describe a fast algorithm for non-negative deconvolution and apply it to readout of LSM detector photocurrents. By broadening photon impulses and deconvolving sampled photocurrent, effective quantum efficiency of the imaging system is increased. Using simulation and imaging with a custom-built two-photon microscope, we demonstrate improved fidelity of images acquired at short dwell times over a wide range of photon rates. Images formed show increased correlation-to-sample equivalent to a 25% increase in photon rate, lower noise, and reduced bleed-through compared to conventional image generation.

Alternate JournalJ Biophotonics
PubMed ID22438321