Bulk Dye Loading for In Vivo Calcium Imaging of Visual Responses in Populations of Tectal Neurons.

TitleBulk Dye Loading for In Vivo Calcium Imaging of Visual Responses in Populations of Tectal Neurons.
Publication TypeJournal Article
Year of Publication2021
AuthorsHogg PW, Haas K
JournalCold Spring Harb Protoc
Date Published2021 Mar 29
ISSN1559-6095
Abstract

Bulk loading of neurons with fluorescent calcium indicators in transparent albino tadpoles offers a rapid and easy method for tracking sensory-evoked activity in large numbers of neurons within an awake developing brain circuit. In vivo two-photon time-lapse imaging of an image plane through the optic tectum allows defining receptive field properties from visual-evoked responses for studies of single-neuron and network-level encoding and plasticity. Here, we describe loading the tadpole optic tectum with the membrane-permeable AM ester of Oregon Green 488 BAPTA-1 (OGB-1 AM) for in vivo imaging experiments.

DOI10.1101/pdb.prot106831
Alternate JournalCold Spring Harb Protoc
PubMed ID33782097