Title | Bulk Dye Loading for In Vivo Calcium Imaging of Visual Responses in Populations of Tectal Neurons. |
Publication Type | Journal Article |
Year of Publication | 2021 |
Authors | Hogg PW, Haas K |
Journal | Cold Spring Harb Protoc |
Date Published | 2021 Mar 29 |
ISSN | 1559-6095 |
Abstract | Bulk loading of neurons with fluorescent calcium indicators in transparent albino tadpoles offers a rapid and easy method for tracking sensory-evoked activity in large numbers of neurons within an awake developing brain circuit. In vivo two-photon time-lapse imaging of an image plane through the optic tectum allows defining receptive field properties from visual-evoked responses for studies of single-neuron and network-level encoding and plasticity. Here, we describe loading the tadpole optic tectum with the membrane-permeable AM ester of Oregon Green 488 BAPTA-1 (OGB-1 AM) for in vivo imaging experiments. |
DOI | 10.1101/pdb.prot106831 |
Alternate Journal | Cold Spring Harb Protoc |
PubMed ID | 33782097 |